Author:
Ma Guojia,Song Qijian,Underwood William R.,Zhang Zhiwei,Fiedler Jason D.,Li Xuehui,Qi Lili
Abstract
Abstract
Sunflower (Helianthus annuus L.) production is challenged by different biotic and abiotic stresses, among which downy mildew (DM) is a severe biotic stress that is detrimental to sunflower yield and quality in many sunflower-growing regions worldwide. Resistance against its infestation in sunflower is commonly regulated by single dominant genes. Pl17 and Pl19 are two broad-spectrum DM resistance genes that have been previously mapped to a gene cluster spanning a 3.2 Mb region at the upper end of sunflower chromosome 4. Using a whole-genome resequencing approach combined with a reference sequence-based chromosome walking strategy and high-density mapping populations, we narrowed down Pl17 to a 15-kb region flanked by SNP markers C4_5711524 and SPB0001. A prospective candidate gene HanXRQChr04g0095641 for Pl17 was identified, encoding a typical TNL resistance gene protein. Pl19 was delimited to a 35-kb region and was approximately 1 Mb away from Pl17, flanked by SNP markers C4_6676629 and C4_6711381. The only gene present within the delineated Pl19 locus in the reference genome, HanXRQChr04g0095951, was predicted to encode an RNA methyltransferase family protein. Six and eight SNP markers diagnostic for Pl17 and Pl19, respectively, were identified upon evaluation of 96 diverse sunflower lines, providing a very useful tool for marker-assisted selection in sunflower breeding programs.
Funder
USDA-AMS Specialty Crop Block Grant Programs
USDA-ARS CRIS Project
Publisher
Springer Science and Business Media LLC
Reference43 articles.
1. Ma, G. J., Markell, S. G., Song, Q. J. & Qi, L. L. Genotyping-by-sequencing targeting of a novel downy mildew resistance gene Pl
20 from wild Helianthus argophyllus for sunflower (Helianthus annuus L.). Theor Appl Genet 130, 1519–1529 (2017).
2. Vear, F. Breeding disease-resistant sunflowers. CAB Reviews 12, 1–11 (2017).
3. Vear, F. et al. Origins of major genes for downy mildew resistance in sunflower. In: Proceedings of 17
th International Sunflower Conference. 125–130 (2008).
4. Vincourt, P. et al. Consensus mapping of major resistance genes and independent QTL for quantitative resistance to sunflower downy mildew. Theor Appl Genet 125, 909–920 (2012).
5. Viranyi, F., Gulya, T. J. & Tourieille, D. L. Recent changes in the pathogenic variability of Plasmopara halstedii (sunflower downy mildew) populations from different continents. Helia 38, 149–162 (2015).
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献