Author:
Gan Lian,Zheng Jianwei,Xu Wei-Hua,Lin Jianhao,Liu Jingshu,Zhang Yu,Wu Zizhan,Lv Zhaolin,Jia Youming,Guo Qingqi,Chen Shijun,Liu Chuanhe,Defoirdt Tom,Qin Qiwei,Liu Yiying
Abstract
AbstractThe muscle of aquatic crustaceans is perishable and susceptible to environmental contamination. Vibrio harveyi is a widely occurring pathogen in aquatic animals. Here, bath treatment with a virulent V. harveyi strain (which was added directly in the rearing water to imitate environmental contamination) isolated from the muscle of the whiteleg shrimp, Litopenaeus vannamei, caused the muscle of Li. vannamei to display a whitish-opaque appearance due to microscopic changes including muscle lysis, muscle fiber damage and microbial colonization. When administered orally by incorporating this isolate in feed (which is an imitation of infection via natural route), rather than direct invasion followed by colonization in the muscle, this isolate indirectly stimulated severe muscle necrosis in Li. vannamei via steering the enrichment of two important (human) pathogens, V. cholerae and V. vulnificus, and one environmental bacterium Pseudomonas oleovorans, based on the meta-taxonomic analyses. In addition to the scientifically proven viral diseases, our research proved that bacterial agents are also capable of causing muscle spoilage in crustaceans via changing the microbial composition, and that the crustaceans might be exploited as the wide-spectrum sensitive bio-detector to indicate the extent of microbial contamination.
Funder
Guangdong Marine Economy Promotion Projects (MEPP) Fund
Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams, Department of Agriculture and Rural Affairs of Guangdong Province
Zhuhai Industry-University-Research Cooperation Project of Zhuhai Science and Technology Innovation Bureau
China Agriculture Research System
Scientific Research Start-up Fund of South China Agricultural University
Publisher
Springer Science and Business Media LLC
Cited by
4 articles.
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