Author:
Shimizu Takahiro,Fujii Takuto,Hanita Keisuke,Shinozaki Ryo,Takamura Yusaku,Suzuki Yoshiro,Kageyama Teppei,Kato Mizuki,Nishijo Hisao,Tominaga Makoto,Sakai Hideki
Abstract
AbstractBitterness is an important physiological function in the defense responses to avoid toxic foods. The taste receptor 2 family is well known to mediate bitter taste perception in Type II taste cells. Here, we report that the polycystic kidney disease 2-like 1 (PKD2L1) channel is a novel sensor for the bitter aftertaste in Type III taste cells. The PKD2L1 channel showed rebound activation after the washout of quinine, a bitter tastant, in electrophysiological whole-cell recordings of the PKD2L1-expressing HEK293T cells and Ca2+-imaging analysis of Type III taste cells isolated from wild-type PKD2L1 mice. In the short-term two-bottle preference and lick tests in vivo, the wild-type mice avoided normal water while the PKD2L1-knockout mice preferred normal water after they ingested the quinine-containing water. These results may explain the new mechanism of the quinine-triggered bitter aftertaste perception in Type III taste cells.
Funder
Japan Society for the Promotion of Science
Tamura Science and Technology Foundation
Lotte Foundation
Publisher
Springer Science and Business Media LLC