Colony spreading of the gliding bacterium Flavobacterium johnsoniae in the absence of the motility adhesin SprB

Author:

Sato Keiko,Naya Masami,Hatano Yuri,Kondo Yoshio,Sato Mari,Narita Yuka,Nagano Keiji,Naito Mariko,Nakayama Koji,Sato Chikara

Abstract

AbstractColony spreading of Flavobacterium johnsoniae is shown to include gliding motility using the cell surface adhesin SprB, and is drastically affected by agar and glucose concentrations. Wild-type (WT) and ΔsprB mutant cells formed nonspreading colonies on soft agar, but spreading dendritic colonies on soft agar containing glucose. In the presence of glucose, an initial cell growth-dependent phase was followed by a secondary SprB-independent, gliding motility-dependent phase. The branching pattern of a ΔsprB colony was less complex than the pattern formed by the WT. Mesoscopic and microstructural information was obtained by atmospheric scanning electron microscopy (ASEM) and transmission EM, respectively. In the growth-dependent phase of WT colonies, dendritic tips spread rapidly by the movement of individual cells. In the following SprB-independent phase, leading tips were extended outwards by the movement of dynamic windmill-like rolling centers, and the lipoproteins were expressed more abundantly. Dark spots in WT cells during the growth-dependent spreading phase were not observed in the SprB-independent phase. Various mutations showed that the lipoproteins and the motility machinery were necessary for SprB-independent spreading. Overall, SprB-independent colony spreading is influenced by the lipoproteins, some of which are involved in the gliding machinery, and medium conditions, which together determine the nutrient-seeking behavior.

Funder

Japan Society for the Promotion of Science

Grant-in-Aid for Structural Cell Biology

Ministry of Education, Culture, Sports, Science and Technology

Core Research for Evolutional Science and Technology

Grant-in-Aid for Scientific Research on Innovative Areas ‘Sparse modeling’

Grant-in-Aid from CANON

Strategic Research grant from AIST to NISP

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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