Affiliation:
1. Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin
Abstract
ABSTRACT
Cells of
Flavobacterium johnsoniae
glide rapidly over surfaces by an unknown mechanism. Eight genes required for gliding motility have been described. Complementation of the nonmotile mutant UW102-48 identified another gene,
gldJ
, that is required for gliding.
gldJ
mutants formed nonspreading colonies, and individual cells were completely nonmotile. Like previously described nonmotile mutants,
gldJ
mutants were deficient in chitin utilization and were resistant to bacteriophages that infect wild-type cells. Cell fractionation and labeling studies with [
3
H]palmitate indicated that GldJ is a lipoprotein. Mutations in
gldA
,
gldB
,
gldD
,
gldF
,
gldG
,
gldH
, or
gldI
resulted in normal levels of
gldJ
transcript but decreased levels of GldJ protein. Expression of truncated GldJ protein in wild-type cells resulted in a severe motility defect. GldJ was found in regular bands that suggest the presence of a helical structure within the cell envelope.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
78 articles.
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