Abstract
AbstractGlucoraphanin is a major secondary metabolite found inBrassicaceaevegetables, especially broccoli, and its degradation product sulforaphane plays an essential role in anticancer. The fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets is necessary for future marker-assisted selection strategies. In this study, we utilized a doubled haploid population consisting of 176 lines derived from two inbred lines (86,101 and 90,196) with significant differences in sulforaphane content, coupled with extensive genotypic and phenotypic data from two independent environments. A linkage map consisting of 438 simple sequence repeats markers was constructed, covering a length of 1168.26 cM. A total of 18 QTLs for sulforaphane metabolism in broccoli florets were detected, 10 were detected in 2017, and the other 8 were detected in 2018. The LOD values of all QTLs ranged from 3.06 to 14.47, explaining 1.74–7.03% of the biochemical variation between two years. Finally, 6 QTLs (qSF-C3-1,qSF-C3-2,qSF-C3-3,qSF-C3-5,qSF-C3-6andqSF-C7) were stably detected in more than one environment, each accounting for 4.54–7.03% of the phenotypic variation explained (PVE) and a total of 30.88–34.86% of PVE. Our study provides new insights into sulforaphane metabolism in broccoli florets and marker-assisted selection breeding inBrassica oleraceacrops.
Funder
the National Natural Science Foundation of China
the National Key Research and Development Program of China
the China Agriculture Research System
the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops
the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences
the State Key Laboratory of Vegetable Germplasm Innovation
Publisher
Springer Science and Business Media LLC
Cited by
12 articles.
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