Abstract
AbstractMethods for the analysis of cell secretions at the single-cell level only provide semiquantitative endpoint readouts. Here we describe a microwell array for the real-time spatiotemporal monitoring of extracellular secretions from hundreds of single cells in parallel. The microwell array incorporates a gold substrate with arrays of nanometric holes functionalized with receptors for a specific analyte, and is illuminated with light spectrally overlapping with the device’s spectrum of extraordinary optical transmission. Spectral shifts in surface plasmon resonance resulting from analyte–receptor bindings around a secreting cell are recorded by a camera as variations in the intensity of the transmitted light while machine-learning-assisted cell tracking eliminates the influence of cell movements. We used the microwell array to characterize the antibody-secretion profiles of hybridoma cells and of a rare subset of antibody-secreting cells sorted from human donor peripheral blood mononuclear cells. High-throughput measurements of spatiotemporal secretory profiles at the single-cell level will aid the study of the physiological mechanisms governing protein secretion.
Funder
1- The Taiwan EPFL PhD scholarship
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
1- The Société Académique Vaudoise-Fonds Novartis Consumer Health
1- Biltema Foundation 2- ISREC Foundation 3- The Société Académique Vaudoise-Fonds Novartis Consumer Health
Publisher
Springer Science and Business Media LLC
Subject
Computer Science Applications,Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology
Cited by
18 articles.
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