High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells-Reference-Cited by-同舟云学术

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High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells

Published:2020-01 Issue:1 Volume:4 Page:111-124
ISSN:2157-846X
Container-title:Nature Biomedical Engineering
language:en
Short-container-title:Nat Biomed Eng

Author:

Kim Hui Kwon,Lee Sungtae,Kim Younggwang,Park Jinman,Min Seonwoo,Choi Jae Woo,Huang Tony P.,Yoon Sungroh,Liu David R.^ORCID,Kim Hyongbum Henry^ORCID

Funder

Foundation for the National Institutes of Health

Howard Hughes Medical Institute

National Research Foundation of Korea

Ministry of Health and Welfare

Publisher

Springer Science and Business Media LLC

Subject

Computer Science Applications,Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology

Link

http://www.nature.com/articles/s41551-019-0505-1.pdf

Reference60 articles.

1. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823 (2013).

2. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823–826 (2013).

3. Hwang, W. Y. et al. Efficient genome editing in zebrafish using a CRISPR–Cas system. Nat. Biotechnol. 31, 227–229 (2013).

4. Cho, S. W., Kim, S., Kim, J. M. & Kim, J. S. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat. Biotechnol. 31, 230–232 (2013).

5. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L. A. RNA-guided editing of bacterial genomes using CRISPR–Cas systems. Nat. Biotechnol. 31, 233–239 (2013).

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