Directed evolution by in vitro compartmentalization-Reference-Cited by-同舟云学术

Directed evolution by in vitro compartmentalization

Published:2006-07 Issue:7 Volume:3 Page:561-570
ISSN:1548-7091
Container-title:Nature Methods
language:en
Short-container-title:Nat Methods

Author:

Miller Oliver J,Bernath Kalia,Agresti Jeremy J,Amitai Gil,Kelly Bernard T,Mastrobattista Enrico,Taly Valérie,Magdassi Shlomo,Tawfik Dan S,Griffiths Andrew D

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

Link

http://www.nature.com/articles/nmeth897.pdf

Reference25 articles.

1. Tawfik, D.S. & Griffiths, A.D. Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 16, 652–656 (1998).

2. Mastrobattista, E. et al. High-throughput screening of enzyme libraries: in vitro evolution of a β-galactosidase by fluorescence-activated sorting of double emulsions. Chem. Biol. 12, 1291–1300 (2005).

3. Bernath, K., Magdassi, S. & Tawfik, D.S. Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery. J. Mol. Biol. 345, 1015–1026 (2005).

4. Griffiths, A.D. & Tawfik, D.S. Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. EMBO J. 22, 24–35 (2003).

5. Cohen, H.M., Tawfik, D.S. & Griffiths, A.D. Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization. Protein Eng. Des. Sel. 17, 3–11 (2004).

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