Abstract
Abstract
Mechanical force regulates periodontal ligament cell (PDL) behavior. However, different force types lead to distinct PDL responses. Here, we report that pretreatment with an intermittent compressive force (ICF), but not a continuous compressive force (CCF), promoted human PDL (hPDL) osteogenic differentiation as determined by osteogenic marker gene expression and mineral deposition in vitro. ICF-induced osterix (OSX) expression was inhibited by cycloheximide and monensin. Although CCF and ICF significantly increased extracellular adenosine triphosphate (ATP) levels, pretreatment with exogenous ATP did not affect hPDL osteogenic differentiation. Gene-expression profiling of hPDLs subjected to CCF or ICF revealed that extracellular matrix (ECM)-receptor interaction, focal adhesion, and transforming growth factor beta (TGF-β) signaling pathway genes were commonly upregulated, while calcium signaling pathway genes were downregulated in both CCF- and ICF-treated hPDLs. The TGFB1 mRNA level was significantly increased, while those of TGFB2 and TGFB3 were decreased by ICF treatment. In contrast, CCF did not modify TGFB1 expression. Inhibiting TGF-β receptor type I or adding a TGF-β1 neutralizing antibody attenuated the ICF-induced OSX expression. Exogenous TGF-β1 pretreatment promoted hPDL osteogenic marker gene expression and mineral deposition. Additionally, pretreatment with ICF in the presence of TGF-β receptor type I inhibitor attenuated the ICF-induced mineralization. In conclusion, this study reveals the effects of ICF on osteogenic differentiation in hPDLs and implicates TGF-β signaling as one of its regulatory mechanisms.
Funder
Chulalongkorn University
the Chulalongkorn Academic Advancement into Its 2nd Century Project
the Japan Society for the Promotion of Science under JSPS-RONPAKU Fellowship (FY2018), Japan.
Thailand Research Fund
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Cell Biology,Cellular and Molecular Neuroscience,Immunology
Reference57 articles.
1. Tomokiyo, A. et. al. Detection, characterization, and clinical application of mesenchymsl stem cells in periodontal ligament tissue. Stem. Cell. Int. 2018, 5450768 (2018).
2. Iwata, T., Yamato, M., Ishikawa, I., Ando, T., Okano, T. Tissue engineering in periodontal tissue. Anat. Rec. (Hoboken). 297, 16–25 (2014).
3. Smith, P. C., Martinez, C., Martinez, J. & McCulloch, C. A. Role of fibroblast populations in periodontal wound healing and tissue remodeling. Front. Physiol. 10, 270 (2019).
4. Mason, A. G., Scott, B. J., van der Glas, H. W., Linden, R. W. & Cadden, S. W. Remote noxious stimuli modulate jaw reflexes evoked by activation of periodontal ligament mechanoreceptors in man. Exp. Physiol. 87, 699–706 (2002).
5. Turker, K. S., Sowman, P. F., Tuncer, M., Tucker, K. J. & Brinkworth, R. S. The role of periodontal mechanoreceptors in mastication. Arch. Oral. Biol. 52, 361–364 (2007).
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