Alteration of extracellular matrix proteins in atrophic periodontal ligament of hypofunctional rat molars

Author:

Nan Daneeya Na,Everts Vincent,Ferreira Joao N.,Trachoo Vorapat,Osathanon ThanaphumORCID,Klincumhom NutthaORCID,Pavasant Prasit

Abstract

Abstract Objectives The aim of this study was to investigate the effect of mechanical force on possible dynamic changes of the matrix proteins deposition in the PDL upon in vitro mechanical and in vivo occlusal forces in a rat model with hypofunctional conditions. Materials and methods Intermittent compressive force (ICF) and shear force (SF) were applied to human periodontal ligament stem cells (PDLSCs). Protein expression of collagen I and POSTN was analyzed by western blot technique. To establish an in vivo model, rat maxillary molars were extracted to facilitate hypofunction of the periodontal ligament (PDL) tissue of the opposing mandibular molar. The mandibles were collected after 4-, 8-, and 12-weeks post-extraction and used for micro-CT and immunohistochemical analysis. Results ICF and SF increased the synthesis of POSTN by human PDLSCs. Histological changes in the hypofunctional teeth revealed a narrowing of the PDL space, along with a decreased amount of collagen I, POSTN, and laminin in perivascular structures compared to the functional contralateral molars. Conclusion Our results revealed that loss of occlusal force disrupts deposition of some major matrix proteins in the PDL, underscoring the relevance of mechanical forces in maintaining periodontal tissue homeostasis by modulating ECM composition.

Funder

Ratchadapiseksomphot Endowment fund, Chulalongkorn University and Faculty Research Fund, Faculty of Dentistry, Chulalongkorn University

Research Exchange Grant, Faculty of Dentistry, Chulalongkorn University

The NSRF via The Program Management Unit for Human Resources & Institutional Development Research and Innovation

Thailand Science Research and Innovation

Publisher

Springer Science and Business Media LLC

Subject

General Dentistry

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