Selective control of synaptically-connected circuit elements by all-optical synapses

Author:

Prakash MansiORCID,Murphy Jeremy,St Laurent RobynORCID,Friedman Nina,Crespo Emmanuel L.,Bjorefeldt Andreas,Pal AkashORCID,Bhagat Yuvraj,Kauer Julie A.ORCID,Shaner Nathan C.,Lipscombe DianeORCID,Moore Christopher I.ORCID,Hochgeschwender UteORCID

Abstract

AbstractUnderstanding percepts, engrams and actions requires methods for selectively modulating synaptic communication between specific subsets of interconnected cells. Here, we develop an approach to control synaptically connected elements using bioluminescent light: Luciferase-generated light, originating from a presynaptic axon terminal, modulates an opsin in its postsynaptic target. Vesicular-localized luciferase is released into the synaptic cleft in response to presynaptic activity, creating a real-time Optical Synapse. Light production is under experimenter-control by introduction of the small molecule luciferin. Signal transmission across this optical synapse is temporally defined by the presence of both the luciferin and presynaptic activity. We validate synaptic Interluminescence by multi-electrode recording in cultured neurons and in mice in vivo. Interluminescence represents a powerful approach to achieve synapse-specific and activity-dependent circuit control in vivo.

Funder

U.S. Department of Health & Human Services | National Institutes of Health

National Science Foundation

W. M. Keck Foundation

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

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