Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement
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Published:2021-11-01
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ISSN:1087-0156
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Container-title:Nature Biotechnology
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language:en
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Short-container-title:Nat Biotechnol
Author:
Gaurav Kumar, Arora SanuORCID, Silva Paula, Sánchez-Martín Javier, Horsnell RichardORCID, Gao LiangliangORCID, Brar Gurcharn S.ORCID, Widrig Victoria, John Raupp W., Singh NarinderORCID, Wu Shuangye, Kale Sandip M.ORCID, Chinoy CatherineORCID, Nicholson PaulORCID, Quiroz-Chávez JesúsORCID, Simmonds JamesORCID, Hayta SadiyeORCID, Smedley Mark A.ORCID, Harwood WendyORCID, Pearce Suzannah, Gilbert DavidORCID, Kangara Ngonidzashe, Gardener Catherine, Forner-Martínez Macarena, Liu Jiaqian, Yu Guotai, Boden Scott A., Pascucci AttilioORCID, Ghosh SreyaORCID, Hafeez Amber N.ORCID, O’Hara TomORCID, Waites JoshuaORCID, Cheema Jitender, Steuernagel BurkhardORCID, Patpour MehranORCID, Justesen Annemarie FejerORCID, Liu ShuyuORCID, Rudd Jackie C.ORCID, Avni RazORCID, Sharon AmirORCID, Steiner BarbaraORCID, Kirana Rizky PasthikaORCID, Buerstmayr HermannORCID, Mehrabi Ali A.ORCID, Nasyrova Firuza Y., Chayut NoamORCID, Matny OadiORCID, Steffenson Brian J.ORCID, Sandhu NitikaORCID, Chhuneja ParveenORCID, Lagudah EvansORCID, Elkot Ahmed F., Tyrrell SimonORCID, Bian XingdongORCID, Davey Robert P., Simonsen Martin, Schauser Leif, Tiwari Vijay K., Randy Kutcher H., Hucl Pierre, Li Aili, Liu Deng-CaiORCID, Mao LongORCID, Xu StevenORCID, Brown-Guedira GinaORCID, Faris JustinORCID, Dvorak JanORCID, Luo Ming-ChengORCID, Krasileva KseniaORCID, Lux ThomasORCID, Artmeier SusanneORCID, Mayer Klaus F. X.ORCID, Uauy CristobalORCID, Mascher MartinORCID, Bentley Alison R.ORCID, Keller BeatORCID, Poland JesseORCID, Wulff Brande B. H.ORCID
Abstract
AbstractAegilops tauschii, the diploid wild progenitor of the D subgenome of bread wheat, is a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. Here we sequenced 242 Ae. tauschii accessions and compared them to the wheat D subgenome to characterize genomic diversity. We found that a rare lineage of Ae. tauschii geographically restricted to present-day Georgia contributed to the wheat D subgenome in the independent hybridizations that gave rise to modern bread wheat. Through k-mer-based association mapping, we identified discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of hexaploids incorporating diverse Ae. tauschii genomes. Exploiting the genomic diversity of the Ae. tauschii ancestral diploid genome permits rapid trait discovery and functional genetic validation in a hexaploid background amenable to breeding.
Funder
Fulbright Association RCUK | Biotechnology and Biological Sciences Research Council United States Department of Agriculture | National Institute of Food and Agriculture Ministry of Agriculture - Saskatchewan Royal Society Innovationsfonden Kementerian Riset, Teknologi dan Pendidikan Tinggi Department of Biotechnology, Ministry of Science and Technology National Natural Science Foundation of China Chinese Academy of Agricultural Sciences U.S. Department of Agriculture National Science Foundation Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Publisher
Springer Science and Business Media LLC
Subject
Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Reference76 articles.
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