Abstract
AbstractHere we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cells. Using the example of the MYC oncogene, we identify important transcriptional targets and show that E-box mutations at MYC target gene promoters reduce cellular fitness.
Funder
Academy of Finland
Syöpäjärjestöt
Jane ja Aatos Erkon Säätiö
Sigrid Juséliuksen Säätiö
RCUK | Medical Research Council
Cancer Research UK
Publisher
Springer Science and Business Media LLC
Subject
Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Cited by
5 articles.
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