High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler-Reference-Cited by-同舟云学术

High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Published:2023-06-12 Issue: Volume: Page:
ISSN:1087-0156
Container-title:Nature Biotechnology
language:en
Short-container-title:Nat Biotechnol

Author:

Sych Taras,Schlegel Jan,Barriga Hanna M. G.,Ojansivu Miina,Hanke Leo^ORCID,Weber Florian^ORCID,Beklem Bostancioglu R.^ORCID,Ezzat Kariem,Stangl Herbert,Plochberger Birgit^ORCID,Laurencikiene Jurga,El Andaloussi Samir^ORCID,Fürth Daniel,Stevens Molly M.^ORCID,Sezgin Erdinc^ORCID

Abstract

AbstractWe introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

https://www.nature.com/articles/s41587-023-01825-5.pdf

Reference21 articles.

1. Axmann, M. et al. Plasma membrane lipids: an important binding site for all lipoprotein classes. Membranes 11, 882 (2021).

2. Kao, C.-Y. & Papoutsakis, E. T. Extracellular vesicles: exosomes, microparticles, their parts, and their targets to enable their biomanufacturing and clinical applications. Curr. Opin. Biotechnol. 60, 89–98 (2019).

3. Hou, X., Zaks, T., Langer, R. & Dong, Y. Lipid nanoparticles for mRNA delivery. Nat. Rev. Mater. 6, 1078–1094 (2021).

4. Choi, D. et al. Mapping subpopulations of cancer cell-derived extracellular vesicles and particles by nano-flow cytometry. ACS Nano 13, 10499–10511 (2019).

5. Morales-Kastresana, A. et al. Labeling extracellular vesicles for nanoscale flow cytometry. Sci. Rep. 7, 1878 (2017).

Cited by 12 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The vast majority of nucleic acid-loaded lipid nanoparticles contain cargo;Journal of Colloid and Interface Science;2024-11

2. Highly Sensitive Detection of Tumor Cell-Derived Exosomes Using Solid-State Nanopores Assisted with a Slight Salt Gradient;ACS Applied Materials & Interfaces;2024-09-06

3. Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles;ACS Nano;2024-06-05

4. Label-Free Electrochemical Imaging of Single Extracellular Vesicles;2024-05-21

5. Antibody-displaying extracellular vesicles for targeted cancer therapy;Nature Biomedical Engineering;2024-05-20