Easy and robust electrotransfection protocol for efficient ectopic gene expression and genome editing in human B cells
Author:
Funder
ANRS INSERM
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,Molecular Medicine
Link
https://www.nature.com/articles/s41434-020-00194-x.pdf
Reference19 articles.
1. Zhao N, Qi J, Zeng Z, Parekh P, Chang CC, Tung CH, et al. Transfecting the hard-to-transfect lymphoma/leukemia cells using a simple cationic polymer nanocomplex. J Control Rel. 2012;159:104–10.
2. Meacham JM, Durvasula K, Degertekin FL, Fedorov AG. Physical methods for intracellular delivery. J Lab Autom. 2014;19:1–18.
3. Kaestner L, Scholz A, Lipp P. Conceptual and technical aspects of transfection and gene delivery. Bioorg Med Chem Let. 2015;25:1171–6.
4. Mosier DE. Introduction for “Safety considerations for retroviral vectors: a short review.”. Appl Biosaf. 2016;9:68–75.
5. Glover DJ, Lipps HJ, Jans DA. Towards safe, non-viral therapeutic gene expression in humans. Nat Rev Genet. 2005;6:299–310.
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