Using Single-Turnover Kinetics with Osmotic Stress To Characterize the EcoRV Cleavage Reaction
Author:
Affiliation:
1. The Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, United States
Publisher
American Chemical Society (ACS)
Subject
Biochemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/bi401089y
Reference46 articles.
1. Metal Activation of Enzymes in Nucleic Acid Biochemistry
2. Roles of metal ions in nucleases
3. One is enough: insights into the two-metal ion nuclease mechanism from global analysis and computational studies
4. Type II restriction endonucleases: structure and mechanism
5. On the Divalent Metal Ion Dependence of DNA Cleavage by Restriction Endonucleases of the EcoRI Family
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1. Analysis of deoxyribonuclease activity by conjugation-free fluorescence polarisation in sub-nanolitre droplets;The Analyst;2020
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