Analysis of deoxyribonuclease activity by conjugation-free fluorescence polarisation in sub-nanolitre droplets-Reference-Cited by-同舟云学术

Analysis of deoxyribonuclease activity by conjugation-free fluorescence polarisation in sub-nanolitre droplets

Published:2020 Issue:9 Volume:145 Page:3222-3228
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Choi Jae-Won¹²³⁴⁵^ORCID,Vasamsetti Bala Murali Krishna¹²³⁴,Choo Jaebum⁶⁷⁸⁴^ORCID,Kim Hak Yong¹²³⁴⁵

Affiliation:

1. Department of Biochemistry

2. Chungbuk National University

3. Cheongju 28644

4. Republic of Korea

5. School of Biological Sciences

6. Department of Chemistry

7. Chung-Ang University

8. Seoul 06974

Abstract

Deoxyribonuclease (DNase) assay using ethidium bromide (EtBr) molecules by conjugation-free fluorescence polarisation under visible light in a droplet-based microfluidic chip.

Funder

National Research Foundation of Korea

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AN/C9AN02380A

Reference46 articles.

1. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD

2. Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum.

3. DNase I mediates internucleosomal DNA degradation in human cells undergoing drug-induced apoptosis

4. DNase I: structure, function, and use in medicine and forensic science

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