Cov-MS: A Community-Based Template Assay for Mass-Spectrometry-Based Protein Detection in SARS-CoV-2 Patients

Author:

Van Puyvelde Bart1,Van Uytfanghe Katleen2,Tytgat Olivier13,Van Oudenhove Laurence4,Gabriels Ralf56ORCID,Bouwmeester Robbin56ORCID,Daled Simon1,Van Den Bossche Tim56ORCID,Ramasamy Pathmanaban567ORCID,Verhelst Sigrid1,De Clerck Laura1,Corveleyn Laura1,Willems Sander1,Debunne Nathan8ORCID,Wynendaele Evelien8,De Spiegeleer Bart8ORCID,Judak Peter9,Roels Kris9,De Wilde Laurie9ORCID,Van Eenoo Peter9,Reyns Tim10,Cherlet Marc11,Dumont Emmie12,Debyser Griet12,t’Kindt Ruben12,Sandra Koen12,Gupta Surya56ORCID,Drouin Nicolas13,Harms Amy13,Hankemeier Thomas13ORCID,Jones Donald J. L.14ORCID,Gupta Pankaj15,Lane Dan15,Lane Catherine S.16,El Ouadi Said16,Vincendet Jean-Baptiste16,Morrice Nick16,Oehrle Stuart17,Tanna Nikunj17ORCID,Silvester Steve18,Hannam Sally18,Sigloch Florian C.19ORCID,Bhangu-Uhlmann Andrea19,Claereboudt Jan4,Anderson N. Leigh20,Razavi Morteza20,Degroeve Sven56,Cuypers Lize21,Stove Christophe2ORCID,Lagrou Katrien21,Martens Geert A.22,Deforce Dieter1,Martens Lennart56ORCID,Vissers Johannes P. C.17ORCID,Dhaenens Maarten1ORCID

Affiliation:

1. ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Ghent, Belgium

2. Laboratory of Toxicology, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium

3. Department of Life Science Technologies, Imec, 3000 Leuven, Belgium

4. Waters Corporation, 2600 Antwerp, Belgium

5. VIB-UGent Center for Medical Biotechnology, VIB, 9000 Ghent, Belgium

6. Department of Biomolecular Medicine, Ghent University, 9000 Ghent Belgium

7. Interuniversity Institute of Bioinformatics in Brussels, ULB/VUB, 1050 Brussels, Belgium

8. Drug Quality and Registration Group, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium

9. Doping Control Laboratory, Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium

10. Department of Clinical Chemistry, Ghent University Hospital, 9000 Ghent, Belgium

11. Department of Pharmacology, Toxicology, and Biochemistry, Faculty of Veterinary Medicine, Ghent University 9000 Ghent, Belgium

12. Research Institute for Chromatography (RIC), 8500 Kortrijk, Belgium

13. Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, 2311 G Leiden, The Netherlands

14. Leicester Cancer Research Centre, RKCSB, University of Leicester, U.K., and John and Lucille van Geest Biomarker Facility, Cardiovascular Research Centre, Glenfield Hospital, Leicester LE1 7RH, United Kingdom

15. The Department of Chemical Pathology and Metabolic Diseases, Level 4, Sandringham Building, Leicester Royal Infirmary, Leicester LE1 7RH, United Kingdom

16. AB Sciex, Alderley Park, Macclesfield SK10 4TG, United Kingdom

17. Waters Corporation, Milford, Massachusetts 01757, United States

18. Alderley Analytical, Alderley Park, Macclesfield SK10 4TG, United Kingdom

19. Polyquant GmbH, 93077 Bad Abbach, Germany

20. SISCAPA Assay Technologies, Inc., Washington, D.C. 20009, United States

21. Clinical Department of Laboratory Medicine, UZ Leuven, KU Leuven, 3000 Leuven, Belgium

22. AZ Delta Medical Laboratories, AZ Delta General Hospital, 8800 Roeselare, Belgium

Funder

Fonds Wetenschappelijk Onderzoek

Horizon 2020 Framework Programme

Bijzonder Onderzoeksfonds

Publisher

American Chemical Society (ACS)

Subject

General Medicine

Reference51 articles.

1. OECD. Testing for COVID-19: A way to lift confinement restrictions 2020, May, 1–21; https://www.oecd.org/coronavirus/policy-responses/testing-for-covid-19-a-way-to-lift-confinement-restrictions-89756248/ (accessed March 29, 2021).

2. False-positive COVID-19 results: hidden problems and costs

3. FDA. SARS-CoV-2 Reference Panel Comparative Data; https://www.fda.gov/medical-devices/coronavirus-covid-19-and-medical-devices/sars-cov-2-reference-panel-comparative-data.

4. ASM. ASM Expresses Concern about Coronavirus Test Reagent Shortages, 2020; https://asm.org/Articles/Policy/2020/March/ASM-Expresses-Concern-about-Test-Reagent-Shortages#:text=ASM%20Expresses%20Concern%20About%20Coronavirus%20Test%20Reagent%20Shortages,March%2010%2C%202020&text=Clinical%20microbiologists%20and%20clinical%20labs,the%20response%20to%20COVID%2D19.&text=We%20applaud%20the%20CDC%20for,specimen%20swab%20instead%20of%20two (accessed March 29, 2021).

5. CEBM. Are you infectious if you have a positive PCR test result for COVID-19? 2020; https://www.cebm.net/covid-19/infectious-positive-pcr-test-result-covid-19/ (accessed March 29, 2021).

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