Ultra-Sensitive Detection of the SARS-CoV-2 Nucleocapsid Protein via a Clustered Regularly Interspaced Short Palindromic Repeat/Cas12a-Mediated Immunoassay

Author:

Yin Wen1,Li Leyao1,Yang Yang2,Yang Yuxin3,Liang Ruijing4ORCID,Ma Lixin1ORCID,Dai Junbiao35ORCID,Mao Guobin3,Ma Yingxin3ORCID

Affiliation:

1. State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China

2. Shenzhen Key Laboratory of Pathogen and Immunity, National Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People’s Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China

3. Shenzhen Key Laboratory of Synthetic Genomics, Guangdong Provincial Key Laboratory of Synthetic Genomics, Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

4. Guangdong Key Laboratory of Nanomedicine, CAS-HK Joint Lab of Biomaterials, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

5. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China

Funder

National Key Research and Development Program of China

Shenzhen Municipal Science and Technology Innovation Council

Youth Innovation Promotion Association of the Chinese Academy of Sciences

Science, Technology and Innovation Commission of Shenzhen Municipality

National Natural Science Foundation of China

Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institute of Sythetic Biology

Guangdong Provincial Key Laboratory of Synthetic Genomics, Chinese Academy of Sciences

Publisher

American Chemical Society (ACS)

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