Full Sequencing of CRISPR/Cas9 Single Guide RNA (sgRNA) via Parallel Ribonuclease Digestions and Hydrophilic Interaction Liquid Chromatography–High-Resolution Mass Spectrometry Analysis-Reference-Cited by-同舟云学术

Full Sequencing of CRISPR/Cas9 Single Guide RNA (sgRNA) via Parallel Ribonuclease Digestions and Hydrophilic Interaction Liquid Chromatography–High-Resolution Mass Spectrometry Analysis

Published:2021-10-26 Issue:44 Volume:93 Page:14792-14801
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Goyon Alexandre¹,Scott Brandon¹,Kurita Kenji¹,Crittenden Christopher M.¹,Shaw David²,Lin Andy³,Yehl Peter¹,Zhang Kelly¹^ORCID

Affiliation:

1. Small Molecule Analytical Chemistry, 1 DNA Way, South San Francisco, California 94080, United States

2. Cell Therapy Engineering and Development, 1 DNA Way, South San Francisco, California 94080, United States

3. Technical Development Project and Portfolio Management Genentech, 1 DNA Way, South San Francisco, California 94080, United States

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c03533

Reference32 articles.

1. Characterization of therapeutic oligonucleotides by liquid chromatography

2. Analytical characterization of liposomes and other lipid nanoparticles for drug delivery

3. The COVID-19 vaccine development landscape

4. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

5. Guide RNA modification as a way to improve CRISPR/Cas9-based genome-editing systems

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