<i>In Situ</i> Complexation of sgRNA and Cas12a Improves the Performance of a One-Pot RPA–CRISPR-Cas12 Assay-Reference-Cited by-同舟云学术

In Situ Complexation of sgRNA and Cas12a Improves the Performance of a One-Pot RPA–CRISPR-Cas12 Assay

Published:2024-06-12 Issue:25 Volume:96 Page:10443-10450
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Lesinski Jake M.¹^ORCID,Moragues Thomas¹,Mathur Prerit¹,Shen Yang²^ORCID,Paganini Carolina¹,Bezinge Léonard¹^ORCID,Verberckmoes Bo³,Van Eenooghe Bodine³,Stavrakis Stavros¹^ORCID,deMello Andrew J.¹^ORCID,Richards Daniel A.¹^ORCID

Affiliation:

1. Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zürich, Switzerland

2. Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zürich, Switzerland

3. Faculty of Medicine and Health Sciences, Department of Public Health and Primary Care, Ghent University, De Pintelaan 185, 9000 Gent, Belgium

Funder

Eidgen?ssische Technische Hochschule Z?rich

ETH4D

Publisher

American Chemical Society (ACS)

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.4c01777

Reference24 articles.

1. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

2. Nucleic acid detection with CRISPR-Cas13a/C2c2

3. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

4. SHERLOCK: nucleic acid detection with CRISPR nucleases

5. Cas12a-based one-pot SNP detection with high accuracy

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