Amplification Efficiency and Template Accessibility as Distinct Causes of Rain in Digital PCR: Monte Carlo Modeling and Experimental Validation-Reference-Cited by-同舟云学术

Amplification Efficiency and Template Accessibility as Distinct Causes of Rain in Digital PCR: Monte Carlo Modeling and Experimental Validation

Published:2022-11-03 Issue:45 Volume:94 Page:15781-15789
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Berden Pieter¹²³⁴^ORCID,Wiederkehr Rodrigo S.¹^ORCID,Lagae Liesbet¹²^ORCID,Michiels Jan³⁴^ORCID,Stakenborg Tim¹^ORCID,Fauvart Maarten¹³⁴^ORCID,Van Roy Willem¹^ORCID

Affiliation:

1. Imec, Leuven 3001, Belgium

2. Department of Physics and Astronomy, KU Leuven, Leuven 3001, Belgium

3. Department of Molecular and Microbial Systems, Centre of Microbial and Plant Genetics, KU Leuven, Leuven 3001, Belgium

4. Center for Microbiology, Flanders Institute for Biotechnology, VIB, Leuven 3001, Belgium

Funder

Fonds Wetenschappelijk Onderzoek

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.2c03534

Reference22 articles.

1. Emerging digital PCR technology in precision medicine

2. The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

3. Impact of variance components on reliability of absolute quantification using digital PCR

4. Evaluating Digital PCR for the Quantification of Human Genomic DNA: Accessible Amplifiable Targets

5. Low copy target detection by Droplet Digital PCR through application of a novel open access bioinformatic pipeline, ‘definetherain’

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