Absolute Copy Number from the Statistics of the Quantification Cycle in Replicate Quantitative Polymerase Chain Reaction Experiments
Author:
Affiliation:
1. Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, United States
2. Department of Andrology, University Hospital Hamburg-Eppendorf, 20246 Hamburg, Germany
Funder
Deutsche Forschungsgemeinschaft
Publisher
American Chemical Society (ACS)
Subject
Analytical Chemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.5b00077
Reference31 articles.
1. Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR
2. Comparing real-time quantitative polymerase chain reaction analysis methods for precision, linearity, and accuracy of estimating amplification efficiency
3. Evaluation of qPCR curve analysis methods for reliable biomarker discovery: Bias, resolution, precision, and implications
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