Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase in <i>Saccharomyces cerevisiae</i>-Reference-Cited by-同舟云学术

Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase in Saccharomyces cerevisiae

Published:2023-07-24 Issue:8 Volume:12 Page:2271-2277
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Gossing Michael¹^ORCID,Limeta Angelo²,Skrekas Christos²^ORCID,Wigglesworth Mark³⁴,Davis Andrew⁵,Siewers Verena²⁶,David Florian²

Affiliation:

1. Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, SE-41320 Gothenburg, Sweden

2. Department of Life Sciences, Chalmers University of Technology, SE-41296 Gothenburg, Sweden

3. Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, Alderley Park SK10 2NA, U.K.

4. Alderley Lighthouse Laboratories Ltd., Alderley Park SK10 4TG, Macclesfield, U.K.

5. Discovery Sciences, Biopharmaceutical R&D, AstraZeneca, Cambridge CB2 0AA, U.K.

6. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark

Funder

AstraZeneca

Vetenskapsrådet

VINNOVA

Knut och Alice Wallenbergs Stiftelse

Cancerfonden

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.2c00689

Reference42 articles.

1. Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering

2. In vivo continuous evolution of genes and pathways in yeast

3. Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

4. Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds

5. Targeted Diversification in the S. cerevisiae Genome with CRISPR-Guided DNA Polymerase I

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