Random Chromosomal Integration and Screening Yields E. coli K-12 Derivatives Capable of Efficient Sucrose Utilization
Author:
Affiliation:
1. Chemical Engineering Department, University of Michigan, Ann Arbor, Michigan 48109, United States
2. Department of Biochemistry, University of Michigan, Ann Arbor, Michigan 48109, United States
Funder
University of Michigan Energy Institute - Beyond Carbon Neutral Program
Publisher
American Chemical Society (ACS)
Subject
Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine
Link
https://pubs.acs.org/doi/pdf/10.1021/acssynbio.0c00392
Reference48 articles.
1. Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci
2. Plasmid copy number noise in monoclonal populations of bacteria
3. Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System
4. Site-specific chromosomal integration of large synthetic constructs
5. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
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