Multiplex Single-Nucleotide Microbial Genome Editing Achieved by CRISPR-Cas9 Using 5′-End-Truncated sgRNAs
Author:
Affiliation:
1. Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University, Anseong 17546, Republic of Korea
Funder
National Research Foundation of Korea
Chung-Ang University
Publisher
American Chemical Society (ACS)
Subject
Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine
Link
https://pubs.acs.org/doi/pdf/10.1021/acssynbio.3c00323
Reference17 articles.
1. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
2. RNA-guided editing of bacterial genomes using CRISPR-Cas systems
3. Multiplex genome editing of microorganisms using CRISPR-Cas
4. Mismatch Intolerance of 5′-Truncated sgRNAs in CRISPR/Cas9 Enables Efficient Microbial Single-Base Genome Editing
5. Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs
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1. Efficient CRISPR-Cas12f1-Mediated Multiplex Bacterial Genome Editing via Low-Temperature Recovery;Journal of Microbiology and Biotechnology;2024-06-14
2. The Influence of Homologous Arm Length on Homologous Recombination Gene Editing Efficiency Mediated by SSB/CRISPR-Cas9 in Escherichia coli;Microorganisms;2024-05-29
3. Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering;Journal of Agricultural and Food Chemistry;2024-05-14
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