Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts-Reference-Cited by-同舟云学术

Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts

Published:2022-01-17 Issue:2 Volume:11 Page:732-746
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Batista Angelo Cardoso¹,Levrier Antoine²,Soudier Paul¹²,Voyvodic Peter L.²^ORCID,Achmedov Tatjana³,Reif-Trauttmansdorff Tristan¹,DeVisch Angelique²,Cohen-Gonsaud Martin²,Faulon Jean-Loup¹^ORCID,Beisel Chase L.³⁴,Bonnet Jerome²,Kushwaha Manish¹^ORCID

Affiliation:

1. Université Paris-Saclay, INRAe, AgroParisTech, Micalis Institute, 78352 Jouy-en-Josas, France

2. Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier, 34090 Montpellier, France

3. Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080 Würzburg, Germany

4. Medical Faculty, University of Würzburg, 97080 Würzburg, Germany

Funder

Agence Nationale de la Recherche

H2020 European Research Council

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.1c00448

Reference62 articles.

1. Cell-free gene expression: an expanded repertoire of applications

2. Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins

3. BioBits™ Explorer: A modular synthetic biology education kit

4. Self-replication of DNA by its encoded proteins in liposome-based synthetic cells

5. A partially self-regenerating synthetic cell

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