FlowCal: A User-Friendly, Open Source Software Tool for Automatically Converting Flow Cytometry Data from Arbitrary to Calibrated Units-Reference-Cited by-同舟云学术

FlowCal: A User-Friendly, Open Source Software Tool for Automatically Converting Flow Cytometry Data from Arbitrary to Calibrated Units

Published:2016-05-12 Issue:7 Volume:5 Page:774-780
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Castillo-Hair Sebastian M.¹,Sexton John T.¹,Landry Brian P.¹,Olson Evan J.¹,Igoshin Oleg A.¹²³,Tabor Jeffrey J.¹³

Affiliation:

1. Department of Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005, United States

2. Center for Theoretical Biological Physics, Rice University, 6500 Main Street, Houston, Texas 77005, United States

3. Department of Biosciences, Rice University, 6100 Main Street, Houston, Texas 77005, United States

Funder

Welch Foundation

Division of Molecular and Cellular Biosciences

National Institute of Allergy and Infectious Diseases

Division of Graduate Education

Office of Naval Research

National Science Foundation

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5b00284

Reference29 articles.

1. Schwartz, A.(Flow Cytometry Standards Corporation) (1988) Calibration Method for Flow Cytometry Using Fluorescent Microbeads and Synthesis Thereof. U.S. Patent 4,767,206.

2. Formalization of the MESF unit of fluorescence intensity

3. Quantitative Fluorescence Calibration: a Tool for Assessing the Quality of Data Obtained by Fluorescence Measurements

4. Standardizing flow cytometry: construction of a standardized fluorescence calibration plot using matching spectral calibrators

5. NIST/ISAC standardization study: Variability in assignment of intensity values to fluorescence standard beads and in cross calibration of standard beads to hard dyed beads

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