Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells-Reference-Cited by-同舟云学术

Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells

Published:2021-09-13 Issue:10 Volume:10 Page:2465-2477
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Yu Hanna¹,Khokhlatchev Andrei V.²,Chew Claude³,Illendula Anuradha²,Conaway Mark⁴,Dryden Kelly⁵,Maeda Denicar Lina Nascimento Fabris¹,Rajasekaran Vignesh¹,Kester Mark²⁶,Zeichner Steven L.¹⁷^ORCID

Affiliation:

1. Department of Pediatrics and Child Health Research Institute, University of Virginia, Charlottesville, Virginia 22903, United States

2. Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22903, United States

3. School of Medicine ORCA, Flow Cytometry Core Facility, University of Virginia, Charlottesville, Virginia 22903, United States

4. Department of Public Health Sciences, University of Virginia, Charlottesville, Virginia 22903, United States

5. Department of Molecular Physiology and Biophysics, University of Virginia, Charlottesville, Virginia 22903, United States

6. Director, nanoSTAR Institute, University of Virginia, Charlottesville, Virginia 22903, United States

7. Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, Virginia 22903, United States

Funder

Wallace H. Coulter Foundation

University of Virginia

Pendleton Pediatric Infectious Disease Laboratory

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.1c00375

Reference38 articles.

1. On the road to synthetic life: the minimal cell and genome-scale engineering

2. How to make a minimal genome for synthetic minimal cell

3. Construction of a minimal genome as a chassis for synthetic biology

4. Construction of consecutive deletions of the Escherichia coli chromosome

5. Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome

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