Accurate Determination of Plasmid Copy Number of Flow-Sorted Cells using Droplet Digital PCR-Reference-Cited by-同舟云学术

Accurate Determination of Plasmid Copy Number of Flow-Sorted Cells using Droplet Digital PCR

Published:2014-06-03 Issue:12 Volume:86 Page:5969-5976
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Jahn Michael¹,Vorpahl Carsten¹,Türkowsky Dominique¹,Lindmeyer Martin²,Bühler Bruno²,Harms Hauke¹,Müller Susann¹

Affiliation:

1. Helmholtz-Centre for Environmental Research − UFZ, Permoserstr. 15, 04318 Leipzig, Germany

2. Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Str. 66, 44227 Dortmund, Germany

Funder

European Commission Directorate-General for Research and Innovation

Helmholtz Association

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/ac501118v

Reference50 articles.

1. Bacillus megaterium—from simple soil bacterium to industrial protein production host

2. Plasmid addiction systems: perspectives and applications in biotechnology

3. Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes

4. Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR

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