Toward Metrological Traceability for DNA Fragment Ratios in GM Quantification. 1. Effect of DNA Extraction Methods on the Quantitative Determination of Bt176 Corn by Real-Time PCR

Author:

Corbisier Philippe1,Broothaerts Wim1,Gioria Sabrina1,Schimmel Heinz1,Burns Malcolm1,Baoutina Anna1,Emslie Kerry R.1,Furui Satoshi1,Kurosawa Yasunori1,Holden Marcia J.1,Kim Hyong-Ha1,Lee Yun-mi1,Kawaharasaki Mamoru1,Sin Della1,Wang Jing1

Affiliation:

1. European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440 Geel, Belgium; LGC, Queens Road, Teddington, Middlesex TW11 0LY, United Kingdom; National Measurement Institute, 1 Suakin Street, Pymble, NSW 2073, Australia; National Food Research Institute, GM Analytical Evaluation Team, Kannondai 2-1-12, Tsukuba, Ibaraki 305-8642, Japan; NIST, DNA Measurements Group, 100 Bureau Drive, Gaithersburg, Maryland 20899-8311; Korea Research Institute...

Publisher

American Chemical Society (ACS)

Subject

General Agricultural and Biological Sciences,General Chemistry

Reference30 articles.

1. Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed.Off. J. Eur. Union2003,L 261, 1−23.

2. Regulation (EC) No 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products from GMOs and amending Directive 2001/18/EC.Off. J. Eur. Union2003,L 268, 24−28.

3. Review of Labelling of Genetically Modified (GM) Foods; Australia and New Zealand Food Authority, 2003; 115 pp.

4. ISO 24276:2006(E). Foodstuffsmethods of analysis for the detection of genetically modified organisms and derived productsgeneral requirements and definitions.

5. ISO 21571:2005(E). Foodstuffsmethods of analysis for the detection of genetically modified organisms and derived productsnucleic acid extraction.

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