Affiliation:
1. Department of Biological and Environmental Sciences, Swedish Mariculture Research Center, University of Gothenburg, Box 463, Gothenburg 40530, Sweden
Abstract
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca
2+
) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca
2+
transfer performed by the OME of the Pacific oyster,
Crassostrea gigas,
as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca
2+
transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a
K
m
of 6.2 mM and a
V
max
of 3.3 nM min
−1
. The transcellular transfer of Ca
2+
accounts for approximately 60% of the total Ca
2+
transfer across the OME of
C. giga
s at environmental Ca
2+
concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca
2+
-channels, plasma-membrane Ca
2+
-ATPase and Na
+
/Ca
2+
-exchanger all participate in the transcellular Ca
2+
transfer across the OME and a model for this Ca
2+
transfer is presented and discussed.
Funder
Helge Ax:son Johnsons stiftelse
Herbert & Karin Jacobssons Stiftelse
The Swedish Mariculture Research center, SWEMARC
Marie Curie Initial Training Networks (ITN), “Calcium in a Changing Environment”, CACHE
Subject
General Agricultural and Biological Sciences,General Environmental Science,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
32 articles.
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