Affiliation:
1. INRAE, Université de Tours, ISP, 37380 Nouzilly, France
Abstract
Poultry are the main source of human infection by
Salmonella
. As infected poultry are asymptomatic, identifying infected poultry farms is difficult, thus controlling animal infections is of primary importance. As cell tropism is known to govern disease, our aim was therefore to identify infected host–cell types in the organs of chicks known to be involved in
Salmonella
infection and investigate the role of the three known invasion factors in this process (T3SS-1, Rck and PagN). Chicks were inoculated with wild-type or isogenic fluorescent
Salmonella
Typhimurium mutants via the intracoelomic route. Our results show that liver, spleen, gall bladder and aortic vessels could be foci of infection, and that phagocytic and non-phagocytic cells, including immune, epithelial and endothelial cells, are invaded
in vivo
in each organ. Moreover, a mutant defective for the T3SS-1, Rck and PagN remained able to colonize organs like the wild-type strain and invaded non-phagocytic cells in each organ studied. As the infection of the gall bladder had not previously been described in chicks, invasion of gall bladder cells was confirmed by immunohistochemistry and infection was shown to last several weeks after inoculation. Altogether, for the first time these findings provide insights into cell tropism of
Salmonella
in relevant organs involved in
Salmonella
infection in chicks and also demonstrate that the known invasion factors are not required for entry into these cell types.
Funder
INRAE/Région Centre doctoral Fellowship
Horizon 2020 Framework Programme
Subject
General Biochemistry, Genetics and Molecular Biology,Immunology,General Neuroscience
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