Affiliation:
1. College of Agriculture, Tongren Polytechnic College, Bijiang District, Tongren City, Guizhou 554300, People's Republic of China
2. National and Local Engineering Research Centre for Separation and Purification Ethnic Chinese Veterinary Herbs, Tongren City, Guizhou 554300, People's Republic of China
Abstract
Pseudorabies virus (PRV), the causative agent of Aujeszky's disease, is one of the most harmful pathogens to the pig industry. PRV can infect and kill a variety of mammals. Nevertheless, the underlying pathogenesis related to PRV is still unclear. This study aims to investigate the pathogenesis induced by PRV in a mouse model. The mice infected with the PRV-HLJ strain developed severe clinical manifestations at 36 h post-infection (hpi), and mortality occurred within 48–72 hpi. Hematoxylin-eosin staining and qRT-PCR methods were used to detect the pathological damage and expression of cytokines related to an immune reaction in brain tissue, respectively. The cytokine storms caused by IFN-α, IFN-
β
, TNF-α, IL-1
β
, IL-6 and IL-18 were related to the histopathological changes induced by PRV. This pattern of cytokine secretion depicts an image of typical cytokine storms, characterized by dysregulated secretion of pro-inflammatory cytokines and imbalanced pro-inflammatory and anti-inflammatory responses. In addition, the pyroptosis pathway was also activated by PRV by elevating the expression levels of nod-like receptor protein 3, Caspase-1, Gasdermin-D and interleukin-1
β
/18. These findings provide a way for further understanding the molecular basis in PRV pathogenesis.
Funder
Guizhou Science and Technology Planing Project
Tongren Science and Technology Planing Project
Cited by
14 articles.
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