Author:
Sun Wei,Liu Shanshan,Yan Yi,Wang Qingyan,Fan Yu,Okyere Samuel Kumi
Abstract
AbstractPseudorabies virus (PRV) is an immunosuppressive virus that causes significant damage to the pig industry. This study aimed to investigate the effects of PRV on oxidative stress and apoptotic related in the spleen of mice to provide basis knowledge for further research on the pathogenesis of PRV in mice model. 36 mice were randomly two groups, the control group which only received 200 μL PBS and infection group which was subcutaneously infected with 200 μL of 1 × 103 TCID50/100 μL PRV, respectively. Spleen tissues in each group were collected for further experiments at 48, 72, and 96 h post-infection (hpi). Pathological observation was performed by hematoxylin and eosin Y staining. Biochemical and Flow cytometry methods were used to determine the reactive oxygen species profile and apoptosis of the spleen post-infection and apoptosis detection. In addition, q-PCR and Western blot were adopted to measure the apoptotic conditions of the spleen infected with PRV. The results indicated that the reactive oxygen species (ROS) level in the PRV infection group was remarkedly increased (p < 0.01) at a time-dependent pattern. Furthermore, the Malondialdehyde levels in the spleen of mice in the infection group increased (p < 0.01) in a time-dependent mode. However, the activity of Catalase, Superoxide dismutase, and glutathione peroxidase and the content of Glutathione in the infection group were decreased with the control group (p < 0.01) at a time-dependent manner. In addition, the ratio of splenocyte apoptosis in the infection group significantly increased (p < 0.01) in a time-dependent manner. In conclusion, PRV infection causes apoptosis of the spleen via oxidative stress in mice.
Funder
Science and Technology Planning Project
Program of Cultivation high-level innovative Talents in Guizhou Province
Publisher
Springer Science and Business Media LLC
Reference21 articles.
1. Müller, T. et al. Pseudorabies virus in wild swine: A global perspective. Adv. Virol. 156, 1691–1705 (2011).
2. Sehl, J. & Teifke, J. P. Comparative pathology of pseudorabies in different naturally and experimentally infected species-A review. Pathogens 9, 633 (2020).
3. Lai, I. H., Chang, C. D. & Shih, W. L. Apoptosis induction by pseudorabies virus via oxidative stress and subsequent DNA damage signaling. Intervirology 62, 1–8 (2019).
4. Tsikas, D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Anal. Biochem. 524, 13–30 (2016).
5. Valyi-Nagy, T. & Dermody, T. S. Role of oxidative damage in the pathogenesis of viral infections of the nervous system. Histol. Histopathol. 20, 957 (2005).
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