Abstract
The first systematic investigation on the influence of carbon dioxide on micro-organisms was carried ont, by Fränkel (1889) who corrected and correlated a number of earlier observations. Many papers have since been published on this subject and a number of these are reviewed in the discussion, p. 211. In a previous paper (Coyne, 1932) preliminary observations regarding the influence of carbon dioxide on the growth of bacteria were reported. The majority of the organisms examined showed marked inhibition of growth in presence of carbon dioxide. These results, which were obtained by incubation of cultures at 14°-18°. For 4 days, have now been extended and the effect of various proportions of carbon dioxide (5 per cent. to 100 per cent.) at different temperature has been investigated over considerable periods. In addition, the statement that the effect cannot be attributed solely to the ph of the medium has been amply confirmed by further experiments.
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