Crystalline glycoprotein cell walls of algae: their stucture, composition and assembly

Author:

Abstract

Cell walls from various algae within the Chlamydomonaceae display, when negatively stained and examined in the electron microscope, a crystalline lattice component. On the basis of the Fourier transforms of micrographs of the cell wall, the algae have been classified into five classes. Most of the algae examined fall into class II. The two-dimensional repeating morphological unit cell of the wall of each class is described and in each case is a parallelogram whose overall dimensions are all of the same order of magnitude. The implications of the classification for classical taxonomy are discussed. More detailed structural information, to a resolution of about 2.5 nm (based on optical filtering and image enhancement techniques), is presented for two of the cell wall classes. From the evidence of polyacrylamide gel electrophoresis, the cell wall of these algae is constructed primarily from a small number of high molecular mass glycoprotein species. In Chlamydomonas reinhardi it has been shown that the hydroxyproline residues in the wall glycoprotein are cross-linked to short oligosaccharides. Some of the conditions necessary for the complete in vitro reassembly of the cell wall of C. reinhardi are presented and include the presence of a discrete nucleating agent and the presence of a physical surface. Dislocations within the crystalline lattice of the cell walls are described and their role as growth points discussed.

Publisher

The Royal Society

Subject

Industrial and Manufacturing Engineering,General Agricultural and Biological Sciences,General Business, Management and Accounting,Materials Science (miscellaneous),Business and International Management

Cited by 176 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3