EVALUATION OF FLAXSEED OIL AND TRICHODERMA SPECIES AGAINST GREY MOLD DISEASE OF GRAPES

Abstract

Grapes (Vitis vinifera L.) are included in vital crops in the world with respect to its nutritious and economic value. Botrytis grey mold, also called noble rot, harms grapes and wine quality caused by Botrytis cinerea. Other than chemicals Trichoderma species considered as important bio control agents for management of post-harvest diseases. The current study was designed to evaluate Trichoderma species for their Micoparasitic ability using a modification of a pre-colonized plate method. In-vitro efficacy of flaxseed oil against the Botrytis cinerea of grapes were determined using disc diffusion technique with different oil concentrations 0.2%, 0.6%, and 0.8%. In-vivo efficacy of most effective formulated and non-formulated Trichoderma concentrations was determined by applying on grape bunches against Botrytis cinerea. Trichoderma Harzianum is more effective and showed 67.5% growth inhibition than Trichoderma Viridae having 64% growth inhibition while flaxseed oil with 0.2%, o.6% and 0.8% concentrations showed 30.19%, 33.32% and 34.89% growth inhibition against botrytis cinerea respectively. In water-based Trichoderma concentrations (1×10⁸) showed maximum growth inhibition after 6 days that was 76.46%. However, it was observed that the percent disease severity index of the grape fruits in oil-based formulation showed 16 %, 20 %, and 18% after the 9 days of incubation whereas the water-based Trichoderma formulations showed 20%, 22%, 24% respectively  as compared with control. Trichoderma species can be considered as bio-control agent while emulsion oil can be used for better shelf life.