Anti-Tumor Effect and Drug Delivery of Biomimetic Exosomes Nanoplatform Loading with Paclitaxel (PTX) for Treating Lung Adenocarcinoma

Abstract

This study constructed an exosome (Exo) nanomedicine with the ability to actively penetrate into lung tumor tissue, in order to improve the anti-tumor effect of paclitaxel (PTX). For reaching this goal A549 lung adenocarcinoma cells were employed. The exosomes were collected by gradient centrifugation, and the Exo/PTX was prepared after targeted modification. Then the in vitro properties and in vivo tumor inhibitory effects of Exo/PTX were then characterized and evaluated. to conduct in vitro and in vivo study. The prepared Exo/PTX had a particle size of about 100 nm, and had a saucer-like double-layer membrane structure, which had a high encapsulation efficiency and drug loading. in vitro studies have shown that Exo/PTX can be largely taken up by lung cancer cells, thereby enhancing the drug’s effects on promoting apoptosis. The results of in vivo experiments showed that Exo/PTX can effectively inhibit the growth of tumor tissue. The results of this study showed that using exosome for drug delivery of PTX can increase the efficacy and decrease the drug toxicity and side effects.