The Role of miR-132-3p in Inhibiting the Proliferation, Invasion and Metastasis of Colon Cancer by Targeted Regulation of PDGF

Abstract

This study investigated the impact of miR-132-3p on colon cancer cell behavior by targeting PDGF, offering insights into potential approaches for understanding colon cancer development and gene-targeted therapy. The study involved qRT-PCR analysis to assess miR-132-3p expression in colon cancer and adjacent tissues. Overexpression of miR-132-3p in HCT-116 cells was examined through CCK-8 assays for proliferation, flow cytometry for apoptosis, and Transwell/scratch assays for invasion/metastasis. Pearson correlation analysis evaluated miR-132-3p-PDGF relationship, validated via luciferase assays. qRT-PCR/Western blot assessed PDGF mRNA/protein levels. In vivo tumorigenesis assays in nude mice confirmed miR-132-3p’s inhibitory effect on HCT-116 cells. Results showed reduced miR-132-3p expression in colon cancer tissues (vs. adjacent tissues), correlating with decreased cell proliferation and invasion upon miR-132-3p overexpression. Luciferase activity confirmed PDGF as a miR-132-3p target. Additionally, miR-132-3p inhibited tumor growth, while miR-132-3p+PDGF reversed these effects. In conclusion, miR-132-3p regulates PDGF, suppressing colon cancer cell proliferation, invasion, and metastasis, thereby promoting apoptosis. This highlights the potential of miR-132-3p as a therapeutic target for colon cancer treatment.