Effect of miR-222 on the Angiogenesis of Bone Microvascular Endothelial Cells After Repair of Vascular Endothelial Growth Factor with Polylactide-Poly(ethylene glycol)-Polylactide (PELA) Microspheres

Author:

Qin Yuxing1,Zhang Kun1,Sun Tao1,Shen Peifeng1,Wang Haoyu1,Zhang Zhiyong1,Qiangui Ren1

Affiliation:

1. Department of Emergency, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region, 010059, Hohhot, People Republic of China

Abstract

The objectives of this study were to show the effect of miR-222 expression on angiogenesis and the mechanism of angiogenesis differentiation of bone microvascular endothelial cells. For the study, the concentration of VEGF release was detected by ELISA, and cell activity was shown using a CCK-8 (Cell Counting Kit-8). We used western blotting to detect VEGFR, TGF-β, and bFGF expression. The results showed that miR-222 expression by day 14 was significantly lower compared to the expression results for days 3 and 7. Additionally, miR-222 expression on day 7 was significantly lower than on day 3. MiR-222 expression in the control group and PELA group decreased gradually over time. On day 14, miR-222 expression in the VEGF microcapsule scaffold group was at its lowest level. Our conclusions were that the regulation of the angiogenesis of bone microvascular endothelial cells appears to be related to (1) the effect of miR-222 on VEGFR and TGF-β, and (2) how bFGF expression is regulated.

Publisher

American Scientific Publishers

Subject

Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3