Early B Cytokine 1 Improves the Proliferation, Invasion and Migration of Ovarian Cancer Cells by Transcriptional Inhibition of the Expression of Cripto-1

Abstract

Ovarian cancer is a common malignant tumor in obstetrics and gynecology and its pathogenesis is complex. EBF1 expression is significantly decreased in ovarian cancer tissues, but its specific mechanism in ovarian cancer has not been studied. In this study, GEPIA website predicted the expression of EBF1 in ovarian cancer tissues. Expression of EBF1 in ovarian cancer cells was detected by RT-qPCR and western blot. CCK-8 and clone formation assay were used to detect the cell proliferation level. Wound healing and Transwell assays detected the levels of cell invasion and migration. Western blot was used to detect the expression of migration-related proteins. Cell transfection techniques were used to overexpress or reduce the expression levels of EBF1 and Cripto-1. Luciferase assay was used to detect the transcriptional activity of Cripto-1 promoter mutant in ovarian cancer cells. ChIP assay was used to verify the combination of EBF1 and the E1 element of the Cripto-1 promoter. The results showed that the expression of EBF1 was down-regulated in all ovarian cancer cell lines. Overexpression of EBF1 can inhibit the proliferation, invasion and migration of ovarian cancer cells, which is achieved by inhibiting the expression of Cripto-1. Overall, EBF1 improved the malignant progression of ovarian cancer cells by transcriptional inhibition of the expression of Cripto-1.