Bone Marrow Mesenchymal Stem Cells (BMSC)-Derived miR-134 Inhibits Cervical Cancer Metastasis

Abstract

The miRNA had been brand-new hot spot for study on pathogenesis of malignant tumor and seeking prevention strategy. The occurrence and development of tumor could be regulated by Gli1/Snail signaling pathway through Hedgehog channel. Our study intends to discuss the role of miRNA derived from BMSC in HPV. The miR-134 derived from BMSC was analyzed through nano-particles and observed under fluorescence microscope along with analysis of miR-134 expression by RTPCR. The HPV rat model was established to analyze miR-134’s role in HPV metastasis in vivo. The level of miR-134 in the staging of N2–N3 was lower than that in N0–N1 staging and lower in patients with metastatic cervical cancer tissue than patients without distant metastasis. Gli1 level could be targeted by miR-134. miR-134 inhibits HPV proliferation and migration by regulating the Gli1/Snail channel through Hedgehog pathway. The inhibitory effect of miR-134 on HH signal pathway could be reversed by Gli1 overexpression. The rats’ EMT and HPV growth was significantly restrained by miR-134 through silencing of Gli1. In conclusion, the growth of HPV is restrained by miR-134 derived from BMSC by regulating Gli1/Snail pathway through Hedgehog channel.