Establishment of Raman Fingerprints and Localization of Main Components of Radix Angelicas sinensis from Different Origins

Abstract

In this paper, confocal micro-Raman spectrum (CMR) was used to investigate the distribution of main components (ferulic acid and nicotinic acid) in Radix Angelicae sinensis (RAS). The ferulic acid and nicotinic acid were scanned to obtain their Raman spectra of single component; the RAS from different origins were scanned to obtain the Raman spectrum of RAS, and the Raman spectrum was compared with single component. The Raman spectrum of a single component was introduced into the map review to localize ferulic acid and nicotinic acid in RAS. In the results, the RAS from different origins showed strong similarity, with obvious characteristic peaks at 120 cm–1 140 cm–1210 cm–1,480 cm–1 and 1050 cm–1. The characteristic peaks of ferulic acid are mainly concentrated in the spectrum range of 100-1100 cm1 with obvious characteristic peaks around 160 cm150 cm–1 and 1800 cm–1 while the peaks of nicotinic acid mainly concentrated in the spectrum range of 100-1100 cm–1, with obvious characteristic peaks around 120 cm–1, 140 cm–1,810 cm–1and 1050 cm–1 The red and the green color represent the locations of ferulic acid and nicotinic acid, respectively. In conclusion, CMR can be used to establish the Raman fingerprint library of RAS for a quick and accurate identify, and locate the known active components.