The immunological effects against invasive aspergillosis disease on inbred mice after the dietary intake of honey varieties with the determination of diastase and invertase enzyme activities

Abstract

Aspergillus fumigatus is a universal pathogenic mold. Invasive aspergillosis disease can affect persons with weakened immune systems. Natural immunostimulators are very important in improving the immune response in contradiction to various diseases. In the current study four varieties of honey samples (nigella, moringa, sidr, and pumpkin honey) which are coded as NS, MO, SI, and PU honey; were used to evaluate diastase and invertase enzyme activities. Eight groups of mice were fed with the four honey varieties, after injecting half of them with invasive aspergillosis infection and comparing results with the positive and negative control of mice groups. Diastase enzyme activity in honey samples ranged from 15.10 ND by Goth Scale in PU Honey to 23.22 ND by Goth Scale in NS Honey. Invertase enzyme activity in honey samples ranged from 21.99 IN in PU Honey to 26.57 IN in MO Honey. All honey varieties activated the cytokine productions such as IL1β and IL6 cytokines. Honey can act as a killing stimulator against invasive aspergillosis infections due to the presence of neutrophil phagocytosis levels in rats. The survival rates among animals treated with the four honey varieties varied from 12.37 in PU Honey Group+Infection to 26.15 in SI Honey Group+Infection. Honey can positively increase innate immune responses and survival rates, and it may be used as a prophylactic or beneficial agent for several diseases.