Evaluation of MALDI-TOF VITEK®MS and VITEK® 2 system for the identification of Staphylococcus saprophyticus-Reference-Cited by-同舟云学术

Evaluation of MALDI-TOF VITEK®MS and VITEK® 2 system for the identification of Staphylococcus saprophyticus

Published:2018-11 Issue:14 Volume:13 Page:1603-1609
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Martins Katheryne B¹,Ferreira Adriano M²,Mondelli Alessandro L³,Rocchetti Taisa T¹,LR de S da Cunha Maria de¹

Affiliation:

1. Department of Microbiology & Immunology, Botucatu Biosciences Institute, UNESP – Univ. Estadual Paulista, Botucatu, SP, Brazil

2. Laboratory of Microbiology, University Hospital of the Botucatu School of Medicine (HC-FMB), UNESP – Univ. Estadual Paulista, Botucatu, SP, Brasil

3. Department of Internal Medicine, Botucatu School of Medicine University Hospital, UNESP – Univ. Estadual Paulista, Botucatu, SP, Brazil

Abstract

Aim: To compare two identification methods for coagulase-negative staphylococci (CoNS) isolated from patients with urinary tract infections, VITEK® 2 and MALDI-TOF VITEK®MS, with genotypic identification by internal transcribed spacer PCR (ITS-PCR). Results: A total of 217 CoNS isolates were studied. Agreement of the VITEK® 2 system with ITS-PCR was 84.8%, with 98% sensitivity and 100% specificity. Thirty-one of the 33 strains incorrectly identified by VITEK® 2 belonged to the species Staphylococcus saprophyticus. MALDI-TOF VITEK®MS showed an excellent correlation with ITS-PCR since it correctly identified all CoNS isolates. Conclusion: MALDI-TOF VITEK®MS is more accurate than the automated VITEK® 2 system in identifying CoNS isolated from urinary tract infections to species level, particularly urinary isolates of S. saprophyticus.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2018-0195

Reference22 articles.

1. Les staphylocoques comme uropathogènes–fréquence d’isolement chez des malades hospitalisés et sensibilité envers les substances antibactériennes

2. Comparison of Genotypic and Phenotypic Methods for Species-Level Identification of Clinical Isolates of Coagulase-Negative Staphylococci

3. Use of Genotypic Identification by sodA Sequencing in a Prospective Study To Examine the Distribution of Coagulase-Negative Staphylococcus Species among Strains Recovered during Septic Orthopedic Surgery and Evaluate Their Significance

4. Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci

Cited by 8 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Staphylococcus capitis Bloodstream Isolates: Investigation of Clonal Relationship, Resistance Profile, Virulence and Biofilm Formation;Antibiotics;2024-02-01

2. Molecular epidemiology of Acinetobacter baumannii complex causing invasive infections in Korean children during 2001–2020;Annals of Clinical Microbiology and Antimicrobials;2023-05-03

3. Genomic and clinical case characterisation of Staphylococcus haemolyticus isolated from dogs and cats in the United States, including strains with high‐level mupirocin tolerance;Veterinary Dermatology;2023-03-13

4. Performance of MALDI–TOF Mass Spectrometry in the Philippines;Tropical Medicine and Infectious Disease;2021-06-26

5. Study of biofilm formation, structure and antibiotic resistance in Staphylococcus saprophyticus strains causing urinary tract infection in women in Ahvaz, Iran;New Microbes and New Infections;2021-01