Author:
Kim Miyoung,Heo Se Ran,Choi Soon Hee,Kwon Hyelin,Park Jeong Su,Seong Moon-Woo,Lee Do-Hoon,Park Kyoung Un,Song Junghan,Kim Eui-Chong
Abstract
Abstract
Background
Three phenotypic identification systems (MicroScan, VITEK 2, and Crystal GP) were evaluated for their accuracy to identify coagulase-negative staphylococci (CNS). A total of 120 clinical isolates confirmed to be CNS via 16S rRNA sequencing and analysis with the MicroSeq 500 v2.0 database were assessed.
Results
The MicroScan, VITEK 2, and Crystal GP systems correctly identified 82.5%, 87.5%, and 67.5% of the isolates, respectively. Misidentification was the main problem in MicroScan (10.8%) and Crystal GP (23.3%) systems, whereas the main problem of VITEK 2 was low-level discrimination (7.5%).
Conclusion
None of the 3 phenotypic systems tested could accurately and reliably identify CNS at the species level. Further verifications such as biochemical testing or 16S rRNA sequencing together with analysis using a comparable database might be helpful in this regard.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Cited by
43 articles.
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