Utilization of stem cells to model Parkinson's disease – current state and future challenges

Abstract

Restricted access to patients and low availability of disease-affected tissue often limit possibilities of research on neurodegenerative diseases. In vitro culture systems to model neurodegenerative diseases have been in use for several years, but derivation, maintenance and differentiation of the appropriate cell types was often a challenge. The development of human induced pluripotent stem cells (hiPSCs) was a milestone in the field and rapid progress is happening since. In this review, we highlight the requirements for standardized hiPSC based in vitro disease modeling, with a specific focus on Parkinson's disease. We describe requirements that are already fulfilled and point out current limitations and challenges. These include the induction of aging, the creation of a cellular 3D environment and the generation of alternative neural progenitor cell types, which still need improvement.