Zika virus alters DNA methylation status of genes involved in Hippo signaling pathway in human neural progenitor cells

Author:

Kandilya Deepika1,Maskomani Silambarasan1,Shyamasundar Sukanya1,Tambyah Paul Anantharajah2,Shiao Yng Chan3,Lee Regina Ching Hua4,Hande Manoor Prakash5,Mallilankaraman Karthik5,Chu Justin Jang Hann4,Dheen S Thameem1

Affiliation:

1. Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597

2. Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597

3. Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597

4. Department of Microbiology & Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597

5. Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597

Abstract

Aim: This study was aimed to understand if Zika virus (ZIKV) alters the DNA methylome of human neural progenitor cells (hNPCs). Materials & methods: Whole genome DNA methylation profiling was performed using human methylationEPIC array in control and ZIKV infected hNPCs. Results & conclusion: ZIKV infection altered the DNA methylation of several genes such as WWTR1 (TAZ) and RASSF1 of Hippo signaling pathway which regulates organ size during brain development, and decreased the expression of several centrosomal-related microcephaly genes, and genes involved in stemness and differentiation in human neural progenitor cells. Overall, ZIKV downregulated the Hippo signaling pathway genes which perturb the stemness and differentiation process in hNPCs, which could form the basis for ZIKV-induced microcephaly.

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

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